Bwa
Program description:
BWA is a program for aligning sequencing reads against a large reference
genome (e.g. human genome). It has two major components, one for read shorter than 150bp and the other for longer reads.
Versions:
Name: bwa Version: 0.7.10 Platform: x86_64 Category: Applications/Engineering URL http://sourceforge.net/projects/bio-bwa/ Provides: bwa
Below is how to load the module into the software:
Module loading reference
Sample SLURM runtime script:
#!/bin/bash #SBATCH --nodes=1 #SBATCH --ntasks-per-node=4 #SBATCH --mem=4gb #SBATCH --time=01:00:00 # Set paths or load appropriate modules module load bwa # Set the $TMPDIR variable export TMPDIR=$HOME/grant_$SLURM_JOB_ACCOUNT/scratch/$USER/$SLURM_JOB_ID # We set application variables export SCR=${TMPDIR} # We set auxiliary variables INPUT_DIR="input" OUTPUT_DIR="output" OUTPUT_FILE="OUTPUT" # Create a temporary directory mkdir -p ${TMPDIR} # Copy the input data to the directory pointed to by the $TMPDIR variable cp ${SLURM_SUBMIT_DIR}/${INPUT_DIR}/* ${TMPDIR} # Go to the $TMPDIR directory cd $TMPDIR # We do the calculations bwa index dmel-all-chromosome-r5.37.fasta # Footer cat << EOF -------------------------------------------------- --------------- End of calculations [$(date)]. -------------------------------------------------- --------------- EOF # We finish the calculations, copy the contents of the $TMPDIR/output directory # to the directory from which the job was queued. mkdir $SLURM_SUBMIT_DIR/${OUTPUT_DIR} cp -r $TMPDIR/* $SLURM_SUBMIT_DIR/${OUTPUT_DIR}/ # Clear working directory rm -rf $TMPDIR
Below are the files that should be in the input directory.
File:Bwa input.zip